Behenic Acid Monolayer and Bilayer Assemblies- A Study of Concanavalin A (Con A) Adsorption and its Interaction with Dextran Using Surface Plasmon Resonance Spectroscopy and Microscopy

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as well as the interaction between deposited Con A with dextran molecules in the presence of Ca2+ and Mn2+ ions was investigated. The results obtained, show that no significant interaction occurs between the adsorbed Con A and dextran in the case of B.A. bilayer, while a significant binding takes place in the monolayer.